The 2-Minute Rule for thr77 login
The 2-Minute Rule for thr77 login
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in lots of tumors, which leads to chromosome instability, and so might lead to tumorigenesis. This protein was discovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important part in endorsing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually explained. Two additional splice variants were noted but thorough nucleotide sequence info is just not nevertheless offered.
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Blocking peptides are peptides that bind specially to the target antibody and block antibody binding. These peptide typically has the epitope identified via the antibody.
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This tutorial outlines further feasible optimization of cellular and immunoassay parameters to be certain the best possible outcomes are...
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This guide outlines even further achievable optimization of mobile and immunoassay parameters to make sure the absolute best results are...
Antibodies certain to the blocking peptide now not bind towards the epitope to the concentrate on protein. By comparing the staining from your blocked antibody as opposed to the antibody by yourself, you can see which staining is specific.
Target Details Cyclin E belongs on the remarkably conserved cyclin family, whose members are characterised by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins function as regulators of CDK kinases. Different cyclins show distinctive expression and degradation designs which lead towards the temporal coordination of every mitotic party. Cyclin E forms a posh with and capabilities to be a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates in the G1-S stage boundary and is also degraded as cells development as a result of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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